Isolation of nucleic acids

The method of nucleic acid isolation is useful for scientists who want to study genes and genetic material related experiments. Nucleic acid isolation can be done with a wide variety of methods and the right tools and equipment can be bought online in GC Biotech web store. Many brands offer their products to researchers who want to make their project easier. Some of those companies are for example CleanNA, who provides kits for cleaning up nucleic acids, or Bioline who supplies scientists with reagents, or Dynamic Devices who build high-throughput applications.

It’s important to get clean samples. Our products make it easier to get batches with high yields of purified and isolated nucleic acids. Methods like the use of magnetic bead based kits can offer a solution here. Also spin columns that bind the material in a smart way are part of the solution we can provide our customers. View more of our nucleic acid isolation products online at our shop.

The process of nucleic acid isolation

There are generally for steps associated with the isolation of nucleic acids. These are lysing, binding, washing and eluting. In the first step the cell structure is disrupted which results in a lysate. Then the cell structure is broken open as well as the nucleus. Now the nucleic acid isolation needs to take place since that material is freed and mixed with noise such as debris and insolubles. The purification takes place and the lot is washed with buffers and eluted. 

Spin columns

With spin columns nucleic acid isolation is done with help of silica in solid phase or other conditions. In all situations a positively charged silica particle will bind to negatively charged genetic sample like DNA or RNA. Then, during centrifugation, the silica will keep hold of the RNA or DNA. With a buffer solution the nucleic acid isolation can take place by eluting the material.